Methods for Imaging Cell Membranes

This introduction to microscopy techniques explores methods for studying cell membrane properties. Methods for Imaging Cell Membranes is essential for students and researchers alike.

This book serves as a comprehensive introduction to microscopy and biomedical imaging methods, specifically focused on studying the distributions and dynamics of molecules on cell surfaces. Methods for Imaging Cell Membranes aims to equip readers with an in-depth understanding of how contemporary microscopy techniques can elucidate the organization of cell membrane systems. It emphasizes the design of experiments leveraging these methodologies to yield meaningful insights into cellular processes.

Various methods are utilized to investigate cell membrane organization, with microscopy techniques being paramount. These techniques not only map the distributions of molecules on cell surfaces but also assess the biophysical properties of membranes. Fluorescence microscopy is particularly highlighted for its specificity and non-invasive nature, enabling live-cell imaging. The advent of super-resolution fluorescence microscopy has further enhanced the capabilities of this field, breaking previous resolution barriers. Other techniques, such as atomic force microscopy and electron microscopy, are also explored to gain a deeper understanding of membrane organization and properties.

Each chapter within Methods for Imaging Cell Membranes is dedicated to measuring specific properties of cell membranes. The authors discuss various methodologies, weighing their advantages and disadvantages, while also highlighting significant discoveries made possible through these techniques. Topics include measuring membrane protein distributions, dynamics, lipid mapping, and more, making this book an invaluable resource for graduate and upper-level undergraduate students, as well as industry researchers in cell biology, microbiology, microscopy, and medical imaging.